Gujarat holds a very unique position in biodiversity spread because of its geographical situate and spread. Microbes being key components of the lives and a magic tool box of potentials; essentially needs no justification for conservation. It is a stepping stone for the construction of Microbial Bio-resource of gujarat. GGI is collecting rhizosphere soil from semiarid region and from that soil samples microbes are isolated and purified for species level identification. Identified species are then submitted to the Genbank. On this basis, we are studying microbial diversity of rhizosphere soil flora. Till the date we have submitted 7721 different sequences to the Genbank. Microbial associations of microbes in various species are being studied such as from Buchanania lanzan (Charoli), Bamboo, Grass spp.Molecular Marker development of Mango is initiated.